Subcutaneous (SQ) Injection — Standard Technique
- Needle: 29–31G, ½" insulin syringe (100-unit)
- Sites: Abdominal (2" from navel), love handles, posterior upper arm, anterior thigh
- Angle: 45–90° depending on adipose tissue
- Pinch: Pinch 1–2" of skin, inject into the fold, hold 5 seconds, release
- Rotate sites: Minimum 1" from last injection. Prevents lipodystrophy
- No aspiration needed for SQ injections
Intramuscular (IM) Injection — NAD+ & Glutathione
- Needle: 25G, 1" (deltoid) or 1.5" (glute)
- Sites: Deltoid (preferred for small volumes), ventrogluteal (preferred for >1mL)
- Z-track technique: Pull skin laterally, inject, hold 10 sec, release — prevents leakback
- Max volume deltoid: 1mL — larger volumes go ventrogluteal
Reconstitution 101
- Always use bacteriostatic water (bac water), NOT sterile water — bac water contains 0.9% benzyl alcohol preservative allowing multi-dose use for 28 days
- Aim down the side of the vial — never spray directly onto the lyophilized cake
- Gentle swirl — never shake. Let it dissolve (1–5 min). Clear = ready
- Refrigerate after reconstitution — 36–46°F (standard fridge). 28-day expiry once reconstituted
- 1 mL = 100 units on an insulin syringe. This is the conversion that makes everything work
- The math: mg in vial ÷ mL of bac water = concentration (mg/mL). Then: desired dose ÷ concentration × 100 = units to draw
- Watch the walkthrough: Reconstitution Video →
250mcg ≈ 7.5 units · 500mcg ≈ 15 units · 1mg ≈ 30 units
Most forgiving peptide in the formulary. Virtually no side effects at any dose.
The gateway peptide — start here. Body Protection Compound-157. Promotes angiogenesis, tendon repair, gut healing, and endothelial protection. Inject SQ near the injury site for targeted repair, or abdominal for systemic/gut. Oral 500mcg 2x/day for IBD/SIBO. 100+ preclinical studies, no adverse events at any dose. This is the easiest sell to skeptical patients.
1mg = 4 units · 2mg = 8 units
The collagen and hair peptide. Resets dermal gene expression — stimulates collagen, elastin, glycosaminoglycans, and hair growth. SQ for systemic anti-aging, topical for targeted skin. Pairs beautifully with microneedling. Co-supplement zinc 30mg/day. Great upsell for your aesthetics patients.
200mcg = 4 units · 300mcg = 6 units
Inject bedtime on empty stomach (no food 2 hours prior).
The gentle GH secretagogue. Synergizes with natural nocturnal GH pulse — doesn't shut down the axis. Patients report better sleep within 1 week, body composition changes at 6–8 weeks. Ideal for anti-aging patients who aren't ready for HGH. 5 on / 2 off schedule to prevent receptor desensitization.
5mg = 100 units (full syringe from 10mg vial)
For higher doses (10–20mg), use 50mg vial when available.
Mitochondria-targeted cardioprotective peptide. Binds cardiolipin in the inner mitochondrial membrane, stabilizing the electron transport chain and rescuing failing mitochondria. FDA Breakthrough Designation for Barth syndrome. Go-to for cardiac patients, high-oxidative-stress conditions, CKD, and mitochondrial dysfunction. Pairs with NAD+ and MOTS-c for a complete mitochondrial support stack.
Standard: 20 units daily · Acute injury: 30–50 units near site
Hard to mess up. Pre-blended 1:1 ratio — one vial, one injection.
Your #1 repair protocol. BPC-157 (angiogenesis + gut) combined with TB-500 (systemic tissue repair + actin regulation). Inject near the injury for targeted healing, abdominal for systemic. Shoulder, knee, and post-surgical patients love this. 6–8 week cycles. This is the word-of-mouth driver for your practice.
50mcg = 1 unit · 100mcg = 2 units
Very low volume draws — use a 0.3mL (30-unit) insulin syringe for precision.
Master regulator of the HPG axis. Activates GnRH neurons via GPR54 receptor, driving pulsatile LH and FSH release. Restores endogenous testosterone production without suppressing spermatogenesis — more physiologic than SERMs. Emerging alternative to enclomiphene for hypogonadism without fertility impairment. Monitor LH, FSH, Total T, and Free T at 4 and 8 weeks.
2mg = 40 units
FDA-approved (Egrifta) for visceral adipose tissue. Only GHRH analog with Phase 3 data specifically for abdominal fat reduction. Great for the patient who's lost weight on GLP-1 but still has visceral fat. 12–26 week cycles. Inject into abdomen. Note: VAT returns on discontinuation — counsel patients about long-term use expectations.
25 units = 2.5mg · 50 units = 5mg · 75 units = 7.5mg · 100 units = 10mg
| Week | Dose | Draw | Notes |
|---|---|---|---|
| 1–4 | 2.5mg | 25 units | Starting dose — assess GI tolerance |
| 5–8 | 5mg | 50 units | Most patients see meaningful weight loss here |
| 9–12 | 7.5mg | 75 units | Titrate only if plateaued & tolerated |
| 13–16 | 10mg | 100 units | High dose — GI side effects more common |
| 17+ | 15mg max | Use 70mg vial | Rarely needed. Monitor closely |
You already know GLP-1 protocols. Tirzepatide is a dual GIP/GLP-1 agonist — same injection technique as semaglutide. Key difference: it targets both incretin receptors. Patients who plateau on semaglutide often respond to tirzepatide. Always titrate slowly — GI side effects (nausea, constipation) are dose-dependent.
10 units = 1mg · 20 units = 2mg · 30 units = 3mg · 40 units = 4mg
| Week | Dose | Draw | Notes |
|---|---|---|---|
| 1–4 | 1mg | 10 units | Starting dose |
| 5–8 | 2mg | 20 units | Assess response |
| 9–12 | 3mg | 30 units | Most patients land here |
| 13+ | 4mg max | 40 units | Max dose — rarely needed |
This is the next generation beyond tirzepatide. Triple agonist — GLP-1, GIP, and glucagon receptor. Phase 2 data: 24% body weight loss at 48 weeks (vs ~21% tirz). The glucagon component adds thermogenesis. Same injection technique. ALWAYS read the vial label — we carry 5 sizes. Use the 30mg for cleanest math.
250mcg = 10 units · 500mcg = 20 units
Good add-on for GLP-1 patients. HGH fragment (amino acids 177–191) — stimulates lipolysis and inhibits lipogenesis without GH side effects. No blood sugar impact, no hunger suppression. Works differently than GLP-1s — targets fat oxidation directly. 12 weeks on, 4 weeks off cycle. Stack with GLP-1 for patients who want extra fat-loss support without dose escalation.
250mcg = 5 units · 500mcg = 10 units
Loading: 250mcg daily × 2–3 weeks with minimal UV. Maintenance: 250mcg weekly.
Tanning peptide — huge demand in your market. Stimulates melanogenesis (MC1R). Patients get a natural-looking tan without UV damage. Start low — 250mcg daily. Side effects: flushing, nausea (self-limiting), libido increase (MC4R crossover). Monitor moles — refer to derm for any changes. Extremely popular with aesthetics clients.
10 units = 0.5mg · 15 units = 0.75mg · 20 units = 1mg
Start LOW at 10 units. Most patients respond better than expected at lower doses.
FDA-approved as Vyleesi for HSDD. Works centrally via MC4R — not a PDE5 inhibitor. Works for both men and women. Effect lasts 24–72 hours from a single dose. PRN use only — not daily. Max 8 doses/month per FDA labeling. Nausea is the main side effect (~40%, self-limiting). Start with 10 units and titrate. This is the compound patients ask about by name.
25 units = 50mg per injection · 100mg/week total
Our 5mL reconstitution gives cleaner math than standard 10mL.
The longevity staple. NAD+ declines ~50% by age 60. Supports sirtuin activation, DNA repair, mitochondrial function. IM injection is the game-changer — bypasses GI degradation, no IV chair time, patients can come in and out in 5 minutes. Great recurring revenue — patients come 2x/week. Stack with glutathione on same visit (different syringe).
200mg = 20 units · 400mg = 40 units
IV push: dilute in 10–20mL saline, push slowly — cramping if rushed.
Master antioxidant — pairs with everything. Liver detox, skin brightening, immune support. IM injection same visit as NAD+ (different syringe). IV push for higher doses. The "glutathione glow" is real — patients notice skin changes within 2–3 weeks of consistent IM dosing. Easy add-on to any visit.
| Compound | Vial | Bac Water | Concentration | Standard Dose | Units to Draw |
|---|---|---|---|---|---|
| 1. BPC-157 ★ | 10mg | 3mL | 3.33mg/mL | 500mcg daily | 15u |
| 2. GHK-Cu ★ | 50mg | 2mL | 25mg/mL | 1mg daily | 4u |
| 3. CJC/Ipa ★ | 10mg | 2mL | 5mg/mL | 300mcg bedtime | 6u |
| 4. SS-31 ★ | 10mg | 2mL | 5mg/mL | 5mg daily | 100u |
| 5. Wolverine ★ | 20mg | 3mL | 6.67mg/mL | 20 units daily | 20u |
| 6. Kisspeptin ★ | 10mg | 2mL | 5mg/mL | 100mcg 1–2x/day | 2u |
| 7. Tesamorelin ★ | 10mg | 2mL | 5mg/mL | 2mg daily | 40u |
| Tirzepatide | 30mg | 3mL | 10mg/mL | 2.5mg weekly | 25u |
| Retatrutide | 30mg | 3mL | 10mg/mL | 1mg weekly | 10u |
| AOD-9604 | 5mg | 2mL | 2.5mg/mL | 250mcg daily | 10u |
| MT-2 | 10mg | 2mL | 5mg/mL | 250mcg daily | 5u |
| PT-141 | 10mg | 2mL | 5mg/mL | 0.5mg PRN | 10u |
| NAD+ | 1,000mg | 5mL | 200mg/mL | 50mg IM 2x/wk | 25u |
| Glutathione | 600mg | 6mL | 100mg/mL | 200mg IM 2–3x/wk | 20u |