Provider Inservice · Peptide Therapy

Peptide protocols,
simplified.

A clinical quick-reference for your team. Reconstitution math, dosing protocols, injection technique, and patient selection — everything your NP needs to confidently prescribe and inject beyond GLP-1s.

Prepared by Dr. M. Scott Mortensen Supplied by Peptide Pure Date: March 26, 2026
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Injection Fundamentals Reconstitution 101 Your Top 7 Protocols Weight Loss & GLP-1 Aesthetics & Skin Sexual Health IV & Wellness Quick Reference Table
Injection Fundamentals

Subcutaneous (SQ) Injection — Standard Technique

  • Needle: 29–31G, ½" insulin syringe (100-unit)
  • Sites: Abdominal (2" from navel), love handles, posterior upper arm, anterior thigh
  • Angle: 45–90° depending on adipose tissue
  • Pinch: Pinch 1–2" of skin, inject into the fold, hold 5 seconds, release
  • Rotate sites: Minimum 1" from last injection. Prevents lipodystrophy
  • No aspiration needed for SQ injections

Intramuscular (IM) Injection — NAD+ & Glutathione

  • Needle: 25G, 1" (deltoid) or 1.5" (glute)
  • Sites: Deltoid (preferred for small volumes), ventrogluteal (preferred for >1mL)
  • Z-track technique: Pull skin laterally, inject, hold 10 sec, release — prevents leakback
  • Max volume deltoid: 1mL — larger volumes go ventrogluteal

Reconstitution 101

  • Always use bacteriostatic water (bac water), NOT sterile water — bac water contains 0.9% benzyl alcohol preservative allowing multi-dose use for 28 days
  • Aim down the side of the vial — never spray directly onto the lyophilized cake
  • Gentle swirl — never shake. Let it dissolve (1–5 min). Clear = ready
  • Refrigerate after reconstitution — 36–46°F (standard fridge). 28-day expiry once reconstituted
  • 1 mL = 100 units on an insulin syringe. This is the conversion that makes everything work
  • The math: mg in vial ÷ mL of bac water = concentration (mg/mL). Then: desired dose ÷ concentration × 100 = units to draw
  • Watch the walkthrough: Reconstitution Video →
Your Top 7 — Selected Protocols
1. BPC-157
In Stock · 118
Vial
10mg
Bac Water
3mL
Dose
250mcg–1mg SQ daily
Site
Near injury or abdomen
Reconstitution — 3mL Standard · Watch Video →
10mg + 3mL bac water = 3.33mg/mL
250mcg ≈ 7.5 units · 500mcg ≈ 15 units · 1mg ≈ 30 units
Most forgiving peptide in the formulary. Virtually no side effects at any dose.
Provider Notes

The gateway peptide — start here. Body Protection Compound-157. Promotes angiogenesis, tendon repair, gut healing, and endothelial protection. Inject SQ near the injury site for targeted repair, or abdominal for systemic/gut. Oral 500mcg 2x/day for IBD/SIBO. 100+ preclinical studies, no adverse events at any dose. This is the easiest sell to skeptical patients.

⚠ Monitor mood — new onset depression/anxiety warrants dose reduction. Bidirectional coagulation effect — caution with anticoagulants.
2. GHK-Cu (Copper Peptide)
In Stock · 50mg & 100mg
Vials
50mg · 100mg
Bac Water
2mL
Dose SQ
1–2mg daily
Topical
1–2% cream
Reconstitution — 50mg Vial
50mg + 2mL = 25mg/mL
1mg = 4 units · 2mg = 8 units
Provider Notes

The collagen and hair peptide. Resets dermal gene expression — stimulates collagen, elastin, glycosaminoglycans, and hair growth. SQ for systemic anti-aging, topical for targeted skin. Pairs beautifully with microneedling. Co-supplement zinc 30mg/day. Great upsell for your aesthetics patients.

3. CJC-1295 / Ipamorelin
In Stock · 189
Vial
10mg (5/5)
Bac Water
2mL
Dose
200–300mcg SQ bedtime
Cycle
5 on / 2 off · 12–16 wk
Reconstitution
10mg (5+5) + 2mL = 5mg/mL
200mcg = 4 units · 300mcg = 6 units
Inject bedtime on empty stomach (no food 2 hours prior).
Provider Notes

The gentle GH secretagogue. Synergizes with natural nocturnal GH pulse — doesn't shut down the axis. Patients report better sleep within 1 week, body composition changes at 6–8 weeks. Ideal for anti-aging patients who aren't ready for HGH. 5 on / 2 off schedule to prevent receptor desensitization.

4. SS-31 (Elamipretide)
In Stock · 10mg
Vial
10mg
Bac Water
2mL
Dose
5–20mg SQ daily
Cycle
8–12 weeks
Reconstitution
10mg + 2mL = 5mg/mL
5mg = 100 units (full syringe from 10mg vial)
For higher doses (10–20mg), use 50mg vial when available.
Provider Notes

Mitochondria-targeted cardioprotective peptide. Binds cardiolipin in the inner mitochondrial membrane, stabilizing the electron transport chain and rescuing failing mitochondria. FDA Breakthrough Designation for Barth syndrome. Go-to for cardiac patients, high-oxidative-stress conditions, CKD, and mitochondrial dysfunction. Pairs with NAD+ and MOTS-c for a complete mitochondrial support stack.

5. BPC-157 / TB-500 — Wolverine Stack
In Stock · 70
Vial
20mg (10/10)
Bac Water
3mL
Dose
20–50 units SQ daily
Site
Near injury or abdomen
Reconstitution · Watch Video →
20mg (10+10) + 3mL = 6.67mg/mL
Standard: 20 units daily · Acute injury: 30–50 units near site
Hard to mess up. Pre-blended 1:1 ratio — one vial, one injection.
Provider Notes

Your #1 repair protocol. BPC-157 (angiogenesis + gut) combined with TB-500 (systemic tissue repair + actin regulation). Inject near the injury for targeted healing, abdominal for systemic. Shoulder, knee, and post-surgical patients love this. 6–8 week cycles. This is the word-of-mouth driver for your practice.

6. Kisspeptin
In Stock · 10mg
Vial
10mg
Bac Water
2mL
Dose
50–100mcg SQ 1–2x/day
Duration
8–12 weeks
Reconstitution
10mg + 2mL = 5mg/mL
50mcg = 1 unit · 100mcg = 2 units
Very low volume draws — use a 0.3mL (30-unit) insulin syringe for precision.
Provider Notes

Master regulator of the HPG axis. Activates GnRH neurons via GPR54 receptor, driving pulsatile LH and FSH release. Restores endogenous testosterone production without suppressing spermatogenesis — more physiologic than SERMs. Emerging alternative to enclomiphene for hypogonadism without fertility impairment. Monitor LH, FSH, Total T, and Free T at 4 and 8 weeks.

7. Tesamorelin
In Stock · 40
Vial
10mg
Bac Water
2mL
Dose
2mg SQ daily
Site
Abdomen (targets VAT)
Reconstitution
10mg + 2mL = 5mg/mL
2mg = 40 units
Provider Notes

FDA-approved (Egrifta) for visceral adipose tissue. Only GHRH analog with Phase 3 data specifically for abdominal fat reduction. Great for the patient who's lost weight on GLP-1 but still has visceral fat. 12–26 week cycles. Inject into abdomen. Note: VAT returns on discontinuation — counsel patients about long-term use expectations.

Weight Loss & GLP-1
Tirzepatide
In Stock
Vials
10 · 15 · 30 · 70mg
Bac Water
3mL (for 30mg)
Route
SQ weekly
Site
Abdomen or thigh
Reconstitution — 30mg Vial with 3mL
30mg + 3mL bac water = 10mg/mL
25 units = 2.5mg · 50 units = 5mg · 75 units = 7.5mg · 100 units = 10mg
WeekDoseDrawNotes
1–42.5mg25 unitsStarting dose — assess GI tolerance
5–85mg50 unitsMost patients see meaningful weight loss here
9–127.5mg75 unitsTitrate only if plateaued & tolerated
13–1610mg100 unitsHigh dose — GI side effects more common
17+15mg maxUse 70mg vialRarely needed. Monitor closely
Provider Notes

You already know GLP-1 protocols. Tirzepatide is a dual GIP/GLP-1 agonist — same injection technique as semaglutide. Key difference: it targets both incretin receptors. Patients who plateau on semaglutide often respond to tirzepatide. Always titrate slowly — GI side effects (nausea, constipation) are dose-dependent.

⚠ Contraindicated in personal/family hx of medullary thyroid carcinoma or MEN2. Monitor for pancreatitis symptoms. Hold if persistent vomiting.
Retatrutide
In Stock · 15/20/30/60mg
Vials
10·15·20·30·60mg
Bac Water
3mL (for 30mg)
Route
SQ weekly
Key
Triple agonist
Reconstitution — 30mg Vial with 3mL (Cleanest Math)
30mg + 3mL bac water = 10mg/mL
10 units = 1mg · 20 units = 2mg · 30 units = 3mg · 40 units = 4mg
WeekDoseDrawNotes
1–41mg10 unitsStarting dose
5–82mg20 unitsAssess response
9–123mg30 unitsMost patients land here
13+4mg max40 unitsMax dose — rarely needed
Provider Notes

This is the next generation beyond tirzepatide. Triple agonist — GLP-1, GIP, and glucagon receptor. Phase 2 data: 24% body weight loss at 48 weeks (vs ~21% tirz). The glucagon component adds thermogenesis. Same injection technique. ALWAYS read the vial label — we carry 5 sizes. Use the 30mg for cleanest math.

⚠ Same MTC/MEN2 contraindication as all GLP-1s. 10mg vials currently on order — use 30mg vials.
AOD-9604
In Stock · 59
Vial
5mg
Bac Water
2mL
Dose
250–500mcg SQ daily
Timing
Fasted AM
Reconstitution
5mg + 2mL bac water = 2.5mg/mL
250mcg = 10 units · 500mcg = 20 units
Provider Notes

Good add-on for GLP-1 patients. HGH fragment (amino acids 177–191) — stimulates lipolysis and inhibits lipogenesis without GH side effects. No blood sugar impact, no hunger suppression. Works differently than GLP-1s — targets fat oxidation directly. 12 weeks on, 4 weeks off cycle. Stack with GLP-1 for patients who want extra fat-loss support without dose escalation.

Additional Protocols — Aesthetics & Skin
Melanotan-2 (MT-2)
In Stock · 68
Vial
10mg
Bac Water
2mL
Loading
250mcg SQ daily × 2–3 wk
Maintenance
250mcg 1–2x/wk
Reconstitution
10mg + 2mL = 5mg/mL
250mcg = 5 units · 500mcg = 10 units
Loading: 250mcg daily × 2–3 weeks with minimal UV. Maintenance: 250mcg weekly.
Provider Notes

Tanning peptide — huge demand in your market. Stimulates melanogenesis (MC1R). Patients get a natural-looking tan without UV damage. Start low — 250mcg daily. Side effects: flushing, nausea (self-limiting), libido increase (MC4R crossover). Monitor moles — refer to derm for any changes. Extremely popular with aesthetics clients.

⚠ Contraindicated with history of melanoma. Patients must monitor moles and report changes immediately. Nausea common at loading dose — resolves.
Sexual Health
PT-141 (Bremelanotide)
In Stock · 44
Vial
10mg
Bac Water
2mL
Dose
10–20 units SQ PRN
Timing
45–60 min before
Reconstitution
10mg + 2mL = 5mg/mL
10 units = 0.5mg · 15 units = 0.75mg · 20 units = 1mg
Start LOW at 10 units. Most patients respond better than expected at lower doses.
Provider Notes

FDA-approved as Vyleesi for HSDD. Works centrally via MC4R — not a PDE5 inhibitor. Works for both men and women. Effect lasts 24–72 hours from a single dose. PRN use only — not daily. Max 8 doses/month per FDA labeling. Nausea is the main side effect (~40%, self-limiting). Start with 10 units and titrate. This is the compound patients ask about by name.

⚠ Transient BP increase possible. Use with caution in uncontrolled hypertension. Monitor for nausea — take with antiemetic PRN if needed.
IV & Wellness
NAD+
In Stock · 164
Vial
1,000mg
Sterile Water
5mL
Dose
50mg IM 2x/week
Route
IM ONLY — deltoid/glute
Reconstitution
1,000mg + 5mL sterile water = 200mg/mL
25 units = 50mg per injection · 100mg/week total
Our 5mL reconstitution gives cleaner math than standard 10mL.
Provider Notes

The longevity staple. NAD+ declines ~50% by age 60. Supports sirtuin activation, DNA repair, mitochondrial function. IM injection is the game-changer — bypasses GI degradation, no IV chair time, patients can come in and out in 5 minutes. Great recurring revenue — patients come 2x/week. Stack with glutathione on same visit (different syringe).

⚠ IM ONLY — do NOT inject SQ (causes significant injection site pain). Use 25G needle, deltoid preferred. Inject slowly.
Glutathione
In Stock · 600mg
Vial
600mg
Sterile Water
6mL
Dose SQ/IM
200–400mg 2–3x/wk
IV Push
600–2,000mg
Reconstitution
600mg + 6mL = 100mg/mL
200mg = 20 units · 400mg = 40 units
IV push: dilute in 10–20mL saline, push slowly — cramping if rushed.
Provider Notes

Master antioxidant — pairs with everything. Liver detox, skin brightening, immune support. IM injection same visit as NAD+ (different syringe). IV push for higher doses. The "glutathione glow" is real — patients notice skin changes within 2–3 weeks of consistent IM dosing. Easy add-on to any visit.

Quick Reference — All Compounds
Compound Vial Bac Water Concentration Standard Dose Units to Draw
1. BPC-157 ★10mg3mL3.33mg/mL500mcg daily15u
2. GHK-Cu ★50mg2mL25mg/mL1mg daily4u
3. CJC/Ipa ★10mg2mL5mg/mL300mcg bedtime6u
4. SS-31 ★10mg2mL5mg/mL5mg daily100u
5. Wolverine ★20mg3mL6.67mg/mL20 units daily20u
6. Kisspeptin ★10mg2mL5mg/mL100mcg 1–2x/day2u
7. Tesamorelin ★10mg2mL5mg/mL2mg daily40u
Tirzepatide30mg3mL10mg/mL2.5mg weekly25u
Retatrutide30mg3mL10mg/mL1mg weekly10u
AOD-96045mg2mL2.5mg/mL250mcg daily10u
MT-210mg2mL5mg/mL250mcg daily5u
PT-14110mg2mL5mg/mL0.5mg PRN10u
NAD+1,000mg5mL200mg/mL50mg IM 2x/wk25u
Glutathione600mg6mL100mg/mL200mg IM 2–3x/wk20u